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| The Plant Cell Biology Core Lab in the Institute of Plant and Microbial Biology, Academia Sinica, was established in the committee meeting on February 10, 2004. This core lab is dedicated to the research and advancement in plant cell biology. A governing committee oversees general operation of the core lab and potential application of promising new technologies in the study of cell biology, while a manager supervises a small staff in providing technical support, routine services and up keeping of the facilities. Major equipment in the core lab includes transmission and scanning electron microscopes, confocal microscopes, a flow cytometer and various types of optical devices. To better serve members in the Institute interested in using electron microscopes for their researches, a new sample-preparation service has been offered. |
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Committee members:
Haw Dai, Mei-Chu Chung, Chih-Hua Tsou, Wan-Hsing Cheng, Guang-Yuh Jauh, Tien-Shin Yu
Laboratory manager:
Wann-Neng Jane
Assistants:
Mei-Jane Fang, Yi-Chia Chou, Feng-Yi Ma, Huei-Ching Song
Facilities:
- Transmission Electron Microscope (Philips CM100, since1996) with Gatan cryo holder
- Scanning Electron Microscope: (FEI Quanta 200, since 2007) with cryo system (Quorum PP2000TR FEI)
- Confocal Laser Scanning Microscope:
- Zeiss LSM 510 Meta with two photon laser (upright, since 2004), Argon 458/477/488/514 nm; HeNe/543 nm; HeNe/633 nm TiSa 690-1040nm
- Zeiss LSM 510 Meta (upright, since 2007), Argon 458/477/488/514 nm; HeNe/543 nm; HeNe/633 nm
- Zeiss LSM 410 (inverted, since 1993) Argon 488/514 nm, HeNe/543 nm, HeNe/633 nm
- Laser Microdissection and Pressure Catapulting System (Zeiss Palm, since 2008)
- Stereo Fluorescence Microscope (Zeiss Lumar. V12, since 2005)
- Inverted Fluorescence Microscope (Zeiss Axiovert S100, since 1999)
- Upright Fluorescent Microscope (Zeiss Axio Imager Z1, since 2005), with motorized Z-stage acquisition allowing for 3D image collection. DIC optics and 3D reconstruction and deconvolution are available.
- Flow Cytometer (COULTER EPICS Elite ESP, since 1997)
- Flow Cytometer, Cell Lab Quanta SC (Beckman Coulter, since 2008)
- Workstation with offline MetaMorph image analysis & Deconvolution.
- High Pressure Freezer (Lecia EM PACT2, since 2006)
- Freeze Fracture/Etching System (BAL-TEC BAF 060, since 2008)
- Freeze Substitution System (Lecia EMAFS, since 1995; Lecia EMAFS2, since 2007)
- Ultramicrotomes: Lecia EM UC6 (since 2005); Lecia Reichert Ultracut S (since 1996); Reichert-Jung Ultracut E with cryo part (since 1983)
- Freeze Dryer (BAL-TEC MED020 coating system, since 2005)
- Critical Point Dryer (Hitachi HCP-2, since 1997)
- Ion Sputter (Hitachi E-1010, since 2006)
- Carbon Coater (Cressington 208C, since 2006)
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